Structures Containing Polyphosphate in Micrococcus lysodeikticus
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Structures containing polyphosphate in Micrococcus lysodeikticus.
Granular structures containing inorganic polyphosphate were found in Micrococcus lysodeikticus. These structures were isolated by fractionation of the bacterial extract obtained by lysing the organisms with lysozyme. The composition of the fraction which was enriched with these structures was found to be: protein, 24%; lipids, 30%; and polyphosphate, 27%. This fraction also contained small amou...
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1. A method is described for the isolation of pure mesosomal membrane fractions from Micrococcus lysodeikticus. 2. Plasmolysis of cells, before wall digestion, was necessary for effective mesosome release. 3. The effect of mild shearing forces, temperature and time upon the release of mesosomal membrane from protoplasts was investigated. 4. The optimum yield of mesosomal membranes from stable p...
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Field, A. K. (Cornell University, Ithaca, N.Y.) and H. B. Naylor. Induction of lysogenic Micrococcus lysodeikticus. J. Bacteriol. 84:1129-1133. 1962.-Between 50 and 60% of log-phase cells of Micrococcus lysodeikticus strain ML 53-40, lysogenic for N5 bacteriophage, were induced by an optimal dose of ultraviolet light. Induction of dilute cell suspensions caused a subsequent 200- to 800-fold inc...
متن کاملImmunological properties of Micrococcus lysodeikticus membranes.
Membranes of Micrococcus lysodeikticus possess antigens which are distinct from other cellular components such as cytoplasm, ribosomes, and cell walls. Only a few (two to three) components are found when dissociated membranes are examined by immunodiffusion and immunoelectrophoresis techniques. Membranes treated with 0.3% sodium dodecyl sulfate, 0.3% Triton X-100, trypsin, phospholipase A or C,...
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Micrococcus lysodeikticus strain ATCC 4698 was treated with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) according to the method of E. A. Adelberg, M. Mandel, and G. C. C. Chen (Biochem. Biophys. Res. Commun. 18:88, 1965). Before and after exposure to 100 ,ug of NTG per ml in tris(hydroxymethyl)aminomethane-maleic buffer (TM), pH 6, cultures were plated on tryptone, yeast extract, and glucose med...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1968
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.96.2.544-553.1968